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投稿时间：2021-08-18
投稿时间：2021-08-18
中文摘要: 以纤细裸藻为原料，利用超声波辅助法对纤细裸藻多糖进行提取。在单因素试验的基础上，以十二烷基硫酸钠（sodium dodecyl sulfate，SDS）浓度、超声时间以及料液比为自变量，多糖得率为响应值，基于Box-Behnken中心组合原理采用响应面法优化提取工艺。试验结果表明，纤细裸藻多糖的最佳提取工艺为SDS浓度20g/L，料液比1∶20（g/mL），超声时间17 min；在此条件下纤细裸藻多糖的得率为35.28%。所得到的纤细裸藻多糖的吸湿性为19.07%（相对湿度81%），在硅胶环境中的保湿性为46.16%，表明纤细裸藻多糖具有一定的吸湿和保湿性能。
Abstract:Euglena gracilis was used as raw material to extract polysaccharides through ultrasonic assisted method.On the basis of single factor test，the concentration of sodium dodecyl sulfate（SDS），ultrasonic time and solid-liquid ratio were used as independent variables，and the yield of E.gracilis polysaccharides was response value.The Box-Behnken design-response surface methodology was employed to optimize the extraction process.The results showed that the optimum extraction process of E.gracilis polysaccharides was as follows:SDS concentration of 20 g/L，solid-liquid ratio of 1∶20（g/mL），and ultrasonic time of 17 min.Under these conditions，the yield of E.gracilis polysaccharides was 35.28%.The moisture absorption capacity of the obtained polysaccharides was 19.07%（relative humidity 81%），and the moisture retention capacity was 46.16% in silica gel environment，indicating that E.gracilis polysaccharides had certain moisture absorption and retention capacities.
keywords: Euglena gracilis polysaccharide sodium dodecyl sulfate ultrasonic assisted extraction moisture absorption capacity moisture retention capacity
文章编号：202220014 中图分类号： 文献标志码：
基金项目:山东省重点研发计划（2020CXGC010604）
| Author Name | Affiliation |
| GAO Li-wei，ZHAO Xin-jie，ZHAO Xiang-zhong* | School of Food Science and Engineering，Qilu University of Technology（Shandong Academy of Sciences），Jinan 250353，Shandong，China |
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