Abstract:In this experiment，19 strains of Saccharomyces cerevisiae stored in the laboratory were selected as the starting strains.Strain CMY-15-1，which can synthesize selenoglutathione，was successfully obtained by screening sodium selenite resistance，selenium resistance acculturation，diethyl sulfate mutagenesis，and ethylthiocyanine resistance.The organic selenium content of CMY-15-1 reached 2 663.3 μg/g.The optimum fermentation conditions of CMC-15-1 were determined by single factor test by screening out carbon，organic nitrogen，and inorganic nitrogen sources and inorganic salt，and the supplemental amount of sodium selenite was optimized.Plackett-Burman designs were used to screen out the significant components affecting the selenoglutathione production of CMY-15-1.Through the steepest ascent test，the addition amount of components in the medium was determined，and the optimal fermentation medium was obtained by central composite design.The content of selenoglutathione was 33.71 μg/L with peptone 5.46 g/L，sodium selenite 205.9 mg/L，ammonium chloride 9.56 g/L，glucose 35 g/L，yeast 8 g/L，dipotassium hydrogen phosphate 0.5 g/L，magnesium chloride 0.5 g/L，and calcium chloride 0.5 g/L.The yield of Se glutathione after optimization was approximately 3.94 times that before optimization.