Abstract:Zhoushan hairtail was used as raw material，and hairtail protein antibacterial peptide was prepared by compound enzymatic hydrolysis.The effects of temperature，pH value，trypsin，β-lactamase，repeated freezing and thawing，and metal ions on the antibacterial activity of hairtail protein antimicrobial peptides were studied.The results showed that the protein antimicrobial peptide of hairtail had strong thermal stability.The antibacterial activity of hairtail protein antimicrobial peptides reached the highest（45.12%）at pH4.8，and the lowest（40.08%）at pH8.8.Under the action of trypsin and β-lactamase，the original antibacterial activity of the antimicrobial peptide was still maintained.The increase of freeze-thaw times decreased the antibacterial effect of the antimicrobial peptide，but only decreased to 90.28% after 6 times of freezing and thawing.The protein antimicrobial peptide of hairtail had strong stability to Na+，K+，Ca2+，and Mg2+，and its antibacterial activity was basically not affected.However，it showed low tolerance to Zn2+，Fe2+，and Fe3+，and the relative antibacterial effect decreased to 86.25%，76.38%，and 74.97%，respectively.Therefore，the contact of hairtail protein antimicrobial peptides with these ions should be avoided.The antimicrobial peptide of hairtail has good stability under various factors and has a certain application prospect as a natural antibacterial agent.