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投稿时间:2022-02-26
投稿时间:2022-02-26
中文摘要: 为探究火麻籽粕多酚的提取工艺及评价其抗氧化活性,在单因素试验基础上,通过响应面试验优化火麻籽粕中多酚的微波辅助提取工艺,并从DPPH自由基清除能力、羟基自由基清除能力、超氧阴离子自由基清除能力和铁离子还原能力4个方面来评价其抗氧化活性。结果表明,最佳提取工艺条件为乙醇体积分数58% 、微波功率311 W、微波时间3.2 min、微波温度50℃、液料比50∶1(mL/g),在此条件下火麻籽粕多酚实际提取量为5.86 mg/g,与理论提取量相对误差仅为0.34% 。试验所选浓度范围内,相较于VC,火麻籽粕多酚对DPPH自由基、羟基自由基、超氧阴离子自由基的清除能力更强,同时还具有较强的还原能力。
Abstract:This paper aims to investigate the extraction process of polyphenols from hemp seed meal and evaluate the antioxidant activity.Based on the single-factor test,the microwave-assisted extraction process of polyphenols from hemp seed meal was optimized by response surface methodology.The antioxidant activity was evaluated in terms of 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical scavenging ability,hydroxyl radical scavenging ability,superoxide anion radical scavenging ability,and ferric reducing ability.The results showed that the optimal extraction conditions were 58% ethanol volume fraction,311 W microwave power,3.2 min microwave time,50 ℃ microwave temperature,and50∶1(mL/g)of liquid to material ratio,under which the actual extraction amount of polyphenols from hemp seed meal was 5.86 mg/g,with a relative error of 0.34% compared with the theoretical extraction amount.Within the selected concentration range,polyphenols from hemp seed meal had a stronger scavenging ability on DPPH radicals,hydroxyl radicals,and superoxide anion radicals,and had stronger reduction ability compared with VC.
文章编号:202216014 中图分类号: 文献标志码:
基金项目:2018-2020年广西本科高校特色专业及实验实训教学基地(中心)建设项目(桂教高教[2018]52号);广西科技师范学院大学生创新创业训练计划项目(X2021246)
作者 | 单位 |
孙晓波1,2,刘卫萍1,王小明1,张鹏1,2,郭松1,2* | 1.广西科技师范学院食品与生化工程学院,广西 来宾 546199;2.广西科技师范学院壮瑶药品质生物学重点实验室,广西 来宾 546199 |
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