Abstract:This study aimed to investigate the anti-fatigue effects of Lyophyllum decastes polysaccharides in mice.A total of 50 mice were randomly divided into five groups:L.decastes polysaccharides low-dose group[100 mg/（kg·d）]，medium-dose group[200 mg/（kg·d）]，and high-dose group[400 mg/（kg·d）]，positive control group[rhodiola 200 mg/（kg·d）]and blank group（distilled water），ten in each group.After 30 consecutive days of intragastric administration，L.decastes polysaccharides low-，medium-and high-dose groups were compared with the blank group and the positive control group to evaluate the anti-fatigue ability of the mice.The results revealed that compared with the blank group，the L.decastes polysaccharides low-，medium-and high-dose groups had increased exhaustive weight-loading swimming time（P＜0.05），content of blood glucose，liver glycogen and muscle glycogen（P＜0.05），and activities of lactate dehydrogenase in serum（P＜0.05），catalase，glutathione peroxidase and superoxide dismutase，in liver tissue（P＜0.05）and Na+K+-ATPase and Ca2+Mg2+-ATPase（P＜0.05），and decreased content of lactic acid and urea nitrogen（P＜0.05）and malondialdehyde（P＜0.05）.No significant difference was observed in the above indexes between the positive control group and L.decastes polysaccharides groups.This study found that L.decastes polysaccharides could effectively ameliorate exercise fatigue.