本文已被：浏览 1845次 下载 987次
投稿时间：2021-06-15
投稿时间：2021-06-15
中文摘要: 以莲子心为原料，去离子水作为溶媒，采用响应面法优化微波辅助提取莲子心多糖的工艺。利用单因素试验优化AB-8大孔树脂脱色工艺，以DPPH自由基、ABTS+自由基和超氧阴离子自由基清除能力评价莲子心多糖的抗氧化性能。结果表明，微波辅助提取莲子心多糖的最佳提取工艺为微波时间4.5 min、微波功率680 W、液料比28∶1（mL/g），此时多糖得率为（4.84±0.11）%。单因素优化后的大孔树脂脱色工艺为大孔树脂添加量4 g、脱色时间60 min、脱色温度50℃。抗氧化活性试验结果表明，莲子心多糖具有较好的DPPH自由基、ABTS+自由基和超氧阴离子自由基清除能力，IC50值分别为 0.472、0.395、0.686 mg/mL。
Abstract:With Plumula nelumbinis as the raw material and deionized water as solvent，microwave-assisted extraction of polysaccharide from P. nelumbinis was optimized by response surface methodology.The decolorization process of AB-8 macroporous resin was improved by single factor experiment，and the antioxidant properties of P.nelumbinis polysaccharide were evaluated by the ability of scavenging DPPH，ABTS+and superoxide anion free radicals.The results showed that the optimum conditions for microwave-assisted extraction of P.nelumbinis polysaccharide were microwave time and power of 4.5 min and 680 W and liquid-to-solid ratio of 28 ∶1（mL/g），and the yield of polysaccharide under these conditions was（4.84 ± 0.11）%.The decolorization requirements after single factor optimization were the addition amount of AB-8 macroporous resin of 4 g and decolorization time and temperature of 60 min and 50℃.The antioxidant activity assay revealed that P.nelumbinis polysaccharide had good scavenging ability of DPPH，ABTS+and superoxide anion free radicals，with IC50values of 0.472，0.395，0.686 mg/mL，respectively.
keywords: Plumula nelumbinis polysaccharide response surface methodology microwave-assisted extraction antioxidant activity
文章编号：202215015 中图分类号： 文献标志码：
基金项目:广东省重点研发计划项目（2019B020212001）
| 作者 | 单位 |
| 胡罗松1，郑青松2，文雨欣2，王和德1，杨雄志1，翟永贞2，张霞2，3，李冰2，3* | 1.广州酒家集团利口福食品有限公司，广东 广州 511442；2.华南理工大学食品科学与工程学院，广东 广州 510640；3.广东省天然产物绿色加工与产品安全重点实验室，广东 广州 510640 |
引用文本：
津ICP备20000603号-1