Abstract:In this study，80% ethanol solution was used to extract corn silk，and then different polar solvents were used to further extract.The active components of n-butanol fraction（NBF）from corn silk ethanol extract were analyzed by liquid chromatography tandem mass spectrometry（LC-MS/MS）method.The antioxidant activity，digestive enzyme inhibitory activity，and hypoglycemic effect on streptozotocin-induced type 2 diabetic mellitus mice were studied.The results showed that NBF had the highest content of total polyphenols and total flavonoids compared with petroleum ether and ethyl acetate fractions，with the strongest antioxidant activity and certain digestive enzyme inhibitory activity.NBF alleviated the declining trend of the weight of type 2 diabetic mellitus mice，and reduced blood glucose and serum insulin levels.NBF improved glucose tolerance，regulated blood lipid level，and improved the activities of antioxidant enzymes.Histopathological results showed that NBF had cytoprotective effects.Therefore，NBF from corn silk ethanol extract had good hypoglycemic effect.