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投稿时间：2021-12-30
投稿时间：2021-12-30
中文摘要: 通过超声辅助Osborne分级法对新型蛋白质源乙醇梭菌蛋白进行分级提取，得到水溶性蛋白、盐溶性蛋白、醇溶性蛋白和碱溶性蛋白4种蛋白质，蛋白含量的大小顺序为碱溶性蛋白＞水溶性蛋白＞盐溶性蛋白＞醇溶性蛋白，对其中含量最多的碱溶性蛋白的提取条件进行优化，结果表明，碱溶性蛋白的最佳提取条件为提取温度50℃、料液比1∶35（g/mL）、提取时间30 min、NaOH溶液质量分数0.3%。此条件下蛋白得率为75.75%。
中文关键词: 乙醇梭菌蛋白 Osborne分级法 碱溶性蛋白 超声辅助提取 提取条件优化
Abstract:Ultrasound-assisted Osborne fractionation was employed to extract proteins from Clostridium autoethanogenum.A total of 4 kinds of proteins were yielded：water-soluble protein，salt-soluble protein，alcohol-soluble protein and alkali-soluble protein.In terms of content，the 4 types of protein were in the order of alkali-soluble protein＞water-soluble protein＞salt-soluble protein＞alcohol-soluble protein.Then the extraction conditions of the most abundant alkali-soluble protein were optimized as follows：extraction temperature of 50 ℃，material-to-liquid ratio of 1∶35（g/mL），extraction for 30 min，and NaOH solution at mass fraction of 0.3%.Under this condition，the yield of the protein was up to 75.75%.
keywords: Clostridium autoethanogenum Osborne fractionation alkali-soluble protein ultrasound-assisted extraction optimization of extraction conditions
文章编号：202213019 中图分类号： 文献标志码：
基金项目:
| Author Name | Affiliation |
| LIU Ru-ming，ZHANG Zhao-hui* | College of Food Science and Engineering，Ocean Unibersity of China，Qingdao 266005，Shandong，China |
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