Abstract:To study the lipid-lowering effect of walnut green husk on mice with acute hyperlipidemia.The experimental mice were randomly divided into the following groups according to body weight：normal，model，fenofibrate groups，and walnut green husk extract low-dose，medium-dose，high-dose groups.After seven days of continuous administration，each mouse in the model group and walnut green husk extract group was intraperitoneally injected with 0.5 mL 75% yolk milk，and mice in the normal group were injected with the same amount of saline.The mice were then fasted without water for 16 h，after which blood was extracted from the eyeballs.The serum levels of total cholesterol，triglyceride，and low-and high-density lipoprotein cholesterols were determined using the kit.High-performance liquid chromatography-mass spectrometry was used to identify the lipid-lowering components of walnut green husk extract.The results showed that fenofibrate and walnut green husk extract significantly reduced the serum levels of total cholesterol，triglyceride，and low-density lipoprotein cholesterol（P＜0.01）and significantly increased the serum level of high-density lipoprotein cholesterol（P＜0.01）.Sixteen polyphenols were identified from walnut green husk extract by high-performance liquid chromatography-mass spectrometry.The lipid-lowering effect observed in this study may be caused by polyphenols in the walnut green husk extract.