本文已被：浏览 955次 下载 326次
投稿时间：2021-05-21
投稿时间：2021-05-21
中文摘要: 对添加黄芪水提物进行深层发酵的黑木耳菌丝体的生长、活性物质含量和多种抗氧化特性进行研究。结果表明，培养液中黄芪水提物浓度为2.5 mg/mL～10.0 mg/mL时可促进黑木耳菌丝生长，在20.0 mg/mL～30.0 mg/mL时则抑制菌丝生长。添加5.0 mg/mL的黄芪水提物深层发酵后，黑木耳菌丝体中的活性成分含量和抗氧化活性显著增加，其胞外多糖、胞内多糖、总酚酸、总黄酮和总皂苷含量分别为对照组的1.88、1.24、2.65、1.61、1.85倍，DPPH自由基清除率、羟基自由基清除率、还原力和亚铁离子螯合率最高分别为对照组的2.27、1.38、1.58、1.97倍。
Abstract:The biomass，active compound contents，and antioxidative properties of Auricularia heimuer mycelia were investigated following submerged fermentation with supplementation of an aqueous extract of Astragalus membranaceus.The results indicated that provision of the A.membranaceus extract at concentrations between 2.5 mg/mL and 10.0 mg/mL promoted A.heimuer mycelial growth，whereas concentrations between 20.0 mg/mL and 30.0 mg/mL inhibited growth.At a concentration of 5.0 mg/mL，the A.membranaceus-supplemented fermentation substrate significantly promoted the contents of active compounds in A.heimuer mycelia and enhanced antioxidative activities.The contents of exopolysaccharides，intercellular polysaccharides，total phenolic acids，total flavonoids，and total saponins detected in A.heimuer mycelia were 1.88，1.24，2.65，1.61，and1.85times those in the controls，and maximally recorded DPPH scavenging，hydroxyl radical scavenging，and ferrochelatation activities were 2.27，1.38，1.58，and 1.97 times those in the controls，respectively.
keywords: Astragalus membranaceus Auricularia heimuer submerged fermentation active compound antioxidative property
文章编号：202212005 中图分类号： 文献标志码：
基金项目:山西农业大学省部共建有机旱作农业国家重点实验室自主研发项目（202105D121008-1-12）；山西省重点研发计划项目（201803D221011-1）
| 作者 | 单位 |
| 蒙秋霞1，徐全飞2，聂建军2，潘保华2，冯婉君2，牛宇2* | 1.山西农业大学资源环境学院省部共建有机旱作农业国家重点实验室，山西 太原 030031；2.山西农业大学农业经济管理学院，山西 太原 030006 |
引用文本：
津ICP备20000603号-1