Abstract:This paper aims to explore the functional substances in jujube residue and improve the utilization rate of waste.To be specific，the ultrasonic-assisted extraction（UAE）of total triterpenoids in jujube residue was optimized by single factor test and response surface methodology（RSM），with the conventional extraction by oscillation（COE）as the control.The antioxidant activity and the anti-A549 cell proliferation activity of the two extracts were compared.The results showed that the optimal UAE conditions were as follows:ultrasonic temperature of 60 ℃，ultrasonic time of 42 min，liquid-to-solid ratio of 34∶1（mL/g），and 64% ethanol.Under the above conditions，the total triterpenoid content（TTC）was 15.854 mg/g.The antioxidant and anti-proliferation activity of the ultrasonic-assisted extract was both higher than that of the extract yielded by COE.The two triterpenoid extracts of the jujube residue displayed strong DPPH radical-，ABTS+radical-，and hydroxyl radical-scavenging ability，and the ability was dose-dependent.At the concentration of 1 mg/mL，the extracts yielded by UAE and COE showed the strongest anti-proliferation activity，and the cancer cell-inhibiting rate reached 37.62% and 27.18%，respectively.Triterpenoids from jujube residue have strong antioxidant activity and anti-proliferation ability.