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投稿时间：2021-11-10
投稿时间：2021-11-10
中文摘要: 以鱿鱼内脏为原料，以提取时间、提取温度、料液比为3个考察因素，在单因素试验基础上，通过响应面法优化鱿鱼内脏中章鱼胺的提取工艺，并探究章鱼胺体外抗氧化活性。通过高效液相色谱对鱿鱼内脏中章鱼胺进行定性定量分析。结果显示，章鱼胺最佳提取工艺参数：以5%三氯乙酸作为提取剂，提取时间2 h，提取温度34℃，料液比1∶5.2（g/mL），此条件下鱿鱼内脏中章鱼胺得率为6.51 mg/g，与预测值6.25 mg/g较为接近。抗氧化试验研究表明，提取的章鱼胺具有明显的抗氧化活性。
Abstract:Using squid viscera as raw material，the influence of extraction time，extraction temperature，and material-to-liquid ratio on the extraction of octopamine from squid viscera was first investigated by single factor experiment，and then the extraction process was optimized by response surface methodology.Moreover，the in vitro antioxidant activity of octopamine was tested.High performance liquid chromatography was performed for qualitative and quantitative analysis of the octopamine in squid viscera.The results showed that the optimal extraction parameters were 5% trichloroacetic acid as extraction solvent，material-to-liquid ratio of 1∶5.2（g/mL），and extraction for 2 h at 34 ℃.Under these conditions，octopamine yield reached 6.51 mg/g，which was close to the predicted value of 6.25 mg/g.In addition，the extracted octopamine had obvious antioxidant activity.
keywords: squid viscera octopamine response surface methodology extraction technology antioxidant activity
文章编号：202210013 中图分类号： 文献标志码：
基金项目:江苏省海洋科技创新专项项目（HY2018-10）；江苏省“333”工程高层次人才培养资金资助项目（BRA2014111）；连云港市“521高层次人才培养工程”资助项目（LYG52105-2018028）
| 作者 | 单位 |
| 孙月，王磊，胡宇，杨威，许依能，纪登杰，陈丽 | 江苏海洋大学药学院，江苏 连云港 222005；江苏省海洋生物产业技术协同创新中心，江苏 连云港 222005；江苏海洋大学江苏省海洋资源开发研究院，江苏 连云港 222005 |
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