Abstract:To investigate the effect of polyphenol from Rosa roxburghii residue on the lipid metabolism and oxidative stress in high-fat diet mice.The mice were divided into blank group，model group，and polyphenol in R.roxburghii residue low-，medium-and high-dose groups[50、100、200 mg/（kg bw）].The experiment lasted for 60 days.After the experiment，the growth condition，serum lipid metabolism and antioxidant indexes of mice were measured，and the mRNA expression levels of 3-hydroxy-3-methyl glutaryl coenzyme A reductase（HMGCoAr），sterol regulatory element binding protein-1c（SREBP-1c），heme oxygenase 1（HO-1），acetyl CoA carboxylase（ACC），fatty acid synthase（FASN）and nuclearfactor erythroidderived 2-like 2（Nrf2）in liver were detected by real-time fluorescence quantitative polymerase chain reaction（qPCR）.The results showed that polyphenol in R.roxburghii residue decreased the body weight，waist circumference，Lee's index and abdominal fat weight and the content of triglyceride（TG），total cholesterol（TC），low-density lipoprotein cholesterol（LDLC），and malondialdehyde（MDA）and increased the content of high-density lipoprotein cholesterol（HDL-C），catalase（CAT），glutathione（GSH），total superoxide dismutase（T-SOD）and glutathione peroxidase（GSH-Px）in serum of high-fat diet mice.The mRNA expression levels of HMG-CoAr，SREBP-1C，FASN and ACC were down-regulated，and those of Nrf2 and HO-1 were significantly up-regulated（P＜0.05）.The effects of polyphenol in R.roxburghii residue high and medium doses were apparent.In conclusion，polyphenol in R.roxburghii residue improved the mice's antioxidant capacity by regulating the synthesis and decomposition of cholesterol and fatty acids and reduced the fat accumulation in liver and oxidative stress damage caused by long-term high-fat diet.