Abstract:An ultra-performance liquid chromatography-tandem mass spectrometry（UPLC-MS/MS）method was developed for simultaneous determination of gallic acid，protocatechuic acid，p-hydroxybenzoic acid，chlorogenic acid，vanillic acid，p-coumaric acid，ferulic acid，and sinapic acid in Hongmeiren.Furthermore，the distribution and proportions of all 8 phenolic acids in samples were analyzed.The 2 mol/L NaOH was used to hydrolyze the raw material for the extraction of phenolic acids（free，esters）.The extract was then purified by solid-phase extraction column before being separated by Waters ACQUITY UPLC HSS T3 column with mobile phase of acetonitrile-0.01% formic acid solution.They were then determined by UPLC-MS/MS in multiple reaction monitoring（MRM）mode and quantified with the external standard method.The results indicated that all 8phenolic acidshad good linearity（r≥ 0.9991）within concentration range of 5 μg/L-5 000 μg/L，with the detection limits of 0.05 μg/L-0.73 μg/L and quantification limits of 0.17 μg/L-2.44 μg/L.The recoveries were 85.7%-103.9%，and the repeatabilitys were in the range of 0.92%-5.28%.All the 8 phenolic acids were detected in Hongmeiren of 6 origins，and the content of total phenolic acids was in the range of 68.82 μg/g-98.96 μg/g.Among them，the content ferulic acid was the highest（38.02 μg/g-53.79 μg/g），followed by chlorogenic acid （15.84 μg/g-25.90 μg/g）.The proportions of phenolic acids were stable among samples of different origins.The proportions of ferulic acid chlorogenic acid in the total phenolic acids were 51.10%-57.51%and 22.29%-27.86%，respectively.