Abstract:To study the optimal extraction conditions and anti-inflammatory activity of Perilla seed oil from Changbai Mountain.Response surface methodology（RSM）was used to optimize the aqueous enzymatic extraction of Perilla seed oil from Changbai Mountain，and its anti-inflammatory activity against mice was explored.The results showed that the optimal extraction conditions were as follows：solid-to-liquid ratio of 1 ∶10（g/mL），alkaline protease content of 3%，and enzymatic hydrolysis temperature and time of 45℃and 3 h.Under the above conditions，the extraction yield of Perilla seed oil from Changbai Mountain was 76.49%.The capillary permeability test and ear swelling test in mice found that the low，medium and high dose groups of Changbai Mountain Perilla seed oil could significantly inhibit the increase of capillary permeability induced by acetic acid in mic and in all dose groups of Changbai Mountain Perilla seed oil，xylene-induced mice auricle swelling was marked or extremely marked inhibited，indicating that Perilla seed oil from Changbai Mountain had good antiinflammatory activity.