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食品研究与开发:2022,43(8):167-176
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GC-IMS技术与HS-SPME/GC-MS技术分析3种茶叶风味成分
(1.韶关学院英东食品学院,广东 韶关 512005;2.新疆大学生命科学与技术学院,新疆 乌鲁木齐 830046;3.珠海雅富兴源食品工业有限公司,广东 珠海 519000)
Detection of Flavor Components in Three Tea Products by GC-IMS Combined with HS-SPME/GC-MS
(1.Henry Fok College of Food Science&Engineering,Shaoguan University,Shaoguan 512005,Guangdong,China;2.College of Life Sciences&Technology,Xinjiang University,Urumqi 830046,Xinjiang,China;3.Zhuhai Yafuxingyuan Food Industry Co.,Ltd.,Zhuhai 519000,Guangdong,China)
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投稿时间:2021-04-05    
中文摘要: 分别采用气相离子迁移色谱法(gas chromatography-ion mobility spectrometry,GC-IMS)与顶空固相微萃取-气质联用技术(headspace-solid phase microextraction/gas chromatography-mass spectrometry,HS-SPME/GC-MS)对非洲卢旺达绿茶和FBOP1、FBOP两个等级的卢旺达红茶的风味成分进行分析。通过GC-MS图谱、GC-IMS图谱与指纹图谱比较3种茶叶之间的风味成分差异,采用动态主成分分析法(dynamic principal component analysis,DPCA)对风味成分进行聚类分析,最近邻算法评估3种茶叶风味的相似度。HS-SPME/GC-MS结果表明,3种茶叶共含有71种主要风味成分,其中共有风味成分有芳樟醇、水杨酸、咖啡因等16种,FBOP1红茶的独有风味成分与FBOP红茶相似,包含全氟三丁胺、苯乙醛、橙花醇等,绿茶的独有风味成分有橙花叔醇、二十七烷酸、茉莉酮等;而GC-IMS结果显示,3种茶叶含有45种共有风味成分,FBOP1红茶与FBOP红茶两个茶叶的风味成分较为相似,其中包括E-2-辛烯醛、苯乙醛、水杨酸等,绿茶的风味成分为3-戊酮、6-甲基-5-庚烯-2-酮等。HS-SPME/GC-MS技术检测出的多为高含量的10个碳原子以上的大分子风味成分,而GC-IMS检测出的多为低含量的10个碳原子以下的小分子风味成分,两种技术联用可较全面地揭示样品的风味成分组成。
Abstract:The flavor components in green tea and two grades (FBOP1 and FBOP)of black tea from Rwanda were respectively analyzed via gas chromatography-ion mobility spectrometry(GC-IMS)and headspace-solid phase microextraction/gas chromatography-mass spectrometry (HS-SPME/GC-MS).GC-MS,GC-IMS,and fingerprint spectra were used to compare the flavor components among the three tea products.Dynamic principal component analysis(DPCA)was performed to cluster the flavor components,and the nearest-neighbor algorithm was used to evaluate the flavor similarity of the three tea products.The results of HS-SPME/GC-MS showed that the three tea products contained 71 main flavor components,among which the 16 common flavor components were linalool,salicylic acid,caffeine,etc.The unique flavor components were similar between the two grades of black tea,mainly including perfluorotributy lamine,phenylacetaldehyde,and nerol.The unique flavor components of green tea were nerolidol,heptadecanoic acid,jasmone,etc.However,the results of GC-IMS showed that three tea products contained 45 common flavor components,and the two grades of black tea had similar flavor components,including E-2-octenal,phenylacetaldehyde,and salicylic acid.The unique flavor components of green tea were 3-pentanone,6-methyl-5-hepten-2-one,etc.Most of the volatile components detected by HS-SPME/GC-MS were large molecules with more than 10 carbon atoms and high content,while GC-IMS mainly detected small molecules with fewer than 10 carbon atoms and low content.Therefore,the two techniques can be combined to fully reveal the flavor components of the samples.
文章编号:202208023     中图分类号:    文献标志码:
基金项目:非洲茶叶香气成分分析及模块香精调配(2019-2Y0024)
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