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投稿时间：2021-06-02
投稿时间：2021-06-02
中文摘要: 通过气相色谱-质谱联用技术分析鉴定水蒸气蒸馏法提取的喙花姜块茎和根中挥发油的化学成分，再对其主要化合物进行检索分析其安全性。通过4-硝基苯基-β-D-吡喃半乳糖苷（4-nitrophenyl-β-D-galactoside，PNPG）法分析其α-葡萄糖苷酶抑制活性。结果表明，喙花姜块茎和根中挥发油的化学成分大致相似，但仍有部分差异，主要成分均为单萜和倍半萜类，其中桉油精相对含量最高，分别达到48.81%和37.23%。其主要化学成分也具有较好的食用安全性。喙花姜块茎和根中挥发油α-葡萄糖苷酶的抑制活性高于阳性对照阿卡波糖。
Abstract:The chemical components of volatile oil from the tubers and roots of Rhynchanthus beesianus extracted by steam distillation were analyzed and identified by gas chromatography-mass spectrometry.The main compounds were evaluated concerning their safety.An assay using 4-nitrophenyl-β-D-galactoside as substrate analyzed α-glucosidase inhibitory activity.The chemical components of the volatile oil in the tuber and root of R.beesianus were similar，but some differences were evident.The main components were monoterpenes and sesquiterpenes.The relative content of Eucalyptus was the highest，reaching 48.81% and 37.23% respectively.The main chemical components were safe to consume.Effects of volatile oil from tubers and roots of R.beesianus on α-glucosidase inhibitory activity were higher than the acarbose positive control.
keywords: Rhynchanthus beesianus volatile oil gas chromatography-mass spectrometry（GC-MS） composition analysis α-glucosidase inhibition
文章编号：202208022 中图分类号： 文献标志码：
基金项目:2019年海南省重点研发计划项目“黎族药用新增项目蔬菜收集、评价、示范与推广”（ZDYF2019029）；现代农业产业技术体系建设专项资金（CARS-21）
| 作者 | 单位 |
| 祁悦，梅文莉，王雅丽，陈惠琴，戴好富，黄圣卓 | 海南大学林学院，海南 海口 570228；中国热带农业科学院热带生物技术研究所，海南热带农业资源研究院，海南省黎药资源天然产物研究与利用重点实验室，海南 海口 571101 |
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