Abstract:Analysis of the active ingredients and antioxidant and antibacterial properties of Pyracantha fortuneana fruits in different regions of Guizhou provided a basis for their development and utilization.Precolumn derivatization-high performance liquid chromatography （HPLC）was used to determine contents of hydrolyzed amino acids，and the total contents of polyphenols and flavonoids were determined using gallic acid and rutin as standards.The results showed that P.fortuneana seeds contained 17 different amino acids.The total amount of essential amino acids was 13.80 mg/g-52.99 mg/g，the essential amino acids/total amino acids（EAA/TAA）was 0.32-0.49，polyphenol oxidase（PPO）activitywas 10.00U/g-20.00U/g，and superoxide dismutase（SOD）activity was 140.72 U/g-888.56 U/g.Sample had total polyphenol content of 0.89 mg GAE/g-1.82 mg GAE/g and total flavonoid content of 0.70 mg Rutin/g-6.32 mg Rutin/g.The DPPH·，ABTS+·and·OH clearance rates of an ethanol extract of P.fortuneana fruit were 96.15%，99.59% and 30.23%，respectively，and the same was tested against bacteria including Escherichia coli and Bacillus subtilis at a concentration of 50 mg/mL.The extract had a significant（p<0.05）antibacterial effect.These results showed that wild P.fortuneana fruit-rich in amino acids，polyphenols and flavonoids with high antioxidant activity had a significant inhibitory effect on pathogenic bacteria.