Abstract:In order to explore the functional components and in vitro antioxidant capacity of blueberry No.1 powder（B1P），an extract of“Lanmei No.1”was independently developed by China.The total anthocyanin content of B1P was determined by the pH differential method，and the anthocyanin types and contents were determined by ultra-high performance liquid chromatography-tandem mass spectrum （UPLC-MS/MS）.Simultaneously，the antioxidant capacity of B1P at different concentrations in vitro was investigated with VCas the reference.The results showed that the total anthocyanin content of B1P was （443.08±16.40）mg/g.An UPLC-MS/MS method for simultaneous detection of eight anthocyanins，was established.The linearity，which was in the concentration range of 0.5 ng/mL to 50.0 ng/mL，was good，and the correlation coefficients R2were all greater than 0.990 0.Among the eight anthocyanins，the highest anthocyanin content was exhibited by delphinidin glucoside （80.90±0.30）mg/g，and the lowest anthocyanin content was exhibited by peonidin glucoside（6.03±0.02）mg/g.In the experimental concentration range of 10 μg/mL-100 μg/mL，the scavenging abilities of ABTS+·，·OH，and superoxide anion were better than that of positive control VC（p<0.05），and the IC50values of B1P to ABTS+·，·OH，and superoxide anion were（37.81±4.30）μg/mL，（51.33±8.03）μg/mL and（19.88±2.88）μg/mL，respectively.Thus，it was concluded that this blueberry contains a large amount of glucosides with delphinidin and peonidin as aglycones.Further，various anthocyanins co-operate to scavenge free radicals with good antioxidant effect.