Abstract:Carbon quantum dots with excellent fluorescence effect were prepared by a hydrothermal method with L-cysteine and glucose as carbon sources.The structure and fluorescence characteristics of the carbon dots were investigated and characterized by ultraviolet absorption and fluorescence spectra.The effects of streptomycin and solution configuration on fluorescence were studied using carbon quantum dots as the fluorescent probe.Streptomycin had an obvious and stable effect on fluorescence at pH7.4.The best excitation wavelength was 440 nm for 1 mL of carbon quantum dots in a 5 mL volumetric bottle.Solutions kept for 15 min were suitable for detection.Streptomycin ranging from 2×10-9mol/L to 2×10-6mol/L produced a good linear relationship with the fluorescence intensity ratio of carbon quantum dots.The linear equation was y=162 219x+0.812 3，the correlation coefficient was 0.997 2，and the detection limit was 8.62×10-10mol/L.Recovery of streptomycin standard solution was high（98.30%-101.50%）with a small relative standard deviation.