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投稿时间：2021-05-20
投稿时间：2021-05-20
中文摘要: 研究咖啡中4种黄曲霉毒素和杂色曲霉素的快速检测方法并对实际样品进行检测。咖啡样品经酸化的乙腈水溶液超声提取后，通过 QuEChERS（quick，easy，cheap，effective，rugged，safe）技术净化，采用超高效液相色谱-串联质谱法进行定性和定量分析。结果表明，5种真菌毒素在1 μg/L～20 μg/L线性范围内线性关系良好，检出限和定量限分别为0.07 μg/kg～0.4 μg/kg和0.2 μg/kg～1.3 μg/kg。4种黄曲霉毒素和杂色曲霉素的平均加标回收率为88.2%～119.0%（n=6），相对标准偏差为1.9%～13.2%。建立的超高效液相色谱-串联质谱法检测方法能够对咖啡豆及咖啡粉样品中黄曲霉毒素和杂色曲霉素进行快速、准确、可靠的分析。
中文关键词: 超高效液相色谱-串联质谱法 咖啡 黄曲霉毒素 杂色曲霉素 QuEChERS技术
Abstract:Qualitative and quantitative analyses of aflatoxins（AFB1，AFB2，AFG1and AFG2）and sterigmatocystin（STG）in coffee beans and instant coffee were performed.The samples were ground and extracted with an aqueous solution of acidified acetonitrile.The sample extracts were purified using a“quick，easy，cheap，effective，rugged，safe”（QuEChERS）approach.Aflatoxins and STG were qualitatively and quantitatively analyzed by ultra-high performance liquid chromatography-tandem mass spectrometry.Aflatoxin and STG concentrations had a good linear relationship in the range of 1 μg/L-20 μg/L.The limit of detectionand limit of quantification were 0.07 μg/kg-0.4 μg/kg and 0.2 μg/kg-1.3 μg/kg，respectively.The average recovery of aflatoxins and STG was 88.2%-119.0%（n=6），with relative standard deviations of 1.9%-13.2%.This method is simple and quantitatively accurate and is thus suitable for the rapid analysis of aflatoxins and STG in coffee beans and instant coffee.
keywords: ultra-high performance liquid chromatography-tandem massspectrometry coffee aflatoxins sterigmatocystin QuEChERS approach
文章编号：202206018 中图分类号： 文献标志码：
基金项目:国家重点研发计划项目（2017YFC1601103）
| Author Name | Affiliation |
| LI Shuo，LI Li | National Institute of Metrology，Beijing 100029，China；National Institutes for Food and Drug Control，Beijing 100050，China |
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