本文已被：浏览 7161次 下载 594次
投稿时间：2021-02-23
投稿时间：2021-02-23
中文摘要: 以草菇蛋白为原料，以DPPH自由基清除率为评价指标，采用单因素试验和响应面试验优化酶解工艺，探讨酶种类、酶解温度、酶解时间、底物浓度和酶浓度对抗氧化肽活性的影响，并采用Box-Behnken试验设计的响应面（response surfacemethodology，RSM）分析方法优化工艺。结果表明，木瓜蛋白酶为最佳酶，酶解草菇蛋白制备抗氧化肽的最佳工艺条件为酶浓度1.0%、底物浓度2.0%、酶解温度55℃、酶解时间180 min，所得的DPPH自由基清除率为85.16%，与预测值相近。对最佳工艺所得抗氧化肽DPPH自由基清除率、羟基自由基清除率、还原能力、ABTS+自由基清除率进行测定，结果表明其具有较好的抗氧化活性。
Abstract:Using Volvariella volvace protein as raw material，and DPPH free radical scavenging rate as an evaluation index，a single factor experiment and response surface methodology（RSM）were used to optimize the enzymolysis process to prepare antioxidant peptides.The effects of enzyme species，enzymolysis temperature，enzymolysis time，substrate concentration，and enzyme concentration on anti-oxidative peptide activity were studied，and the process was optimized by proresponse surface methodology.Papain was the best enzyme，and optimum conditions for preparation of antioxidant peptides by enzymatic hydrolysis of Volvariella volvace protein were determined to be as follows：1.0% enzyme concentration，2.0% substrate concentration，enzymatic hydrolysis temperature 55 ℃，and enzymolysis time 180 min.Under these conditions，the DPPH radical scavenging rate was 85.16%（close to the predicted value）.DPPH free radical scavenging rate，hydroxyl free radical scavenging rate，reducing capacity，and ABTS+free radical scavenging rate of antioxidant peptides obtained by the optimized process were determined，and results indicated that they displayed good antioxidant activity.
keywords: Volvariella volvace protein antioxidant peptide enzymolysis response surface process optimization
文章编号：202204022 中图分类号： 文献标志码：
基金项目:江苏省高等学校自然科学研究重大项目（18KJA530002）；江苏省凹土资源利用重点实验室开放基金（HPK201906）；淮阴工学院研究生科技创新计划项目（HGYK202007）
引用文本：
津ICP备20000603号-1