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食品研究与开发:2022,43(3):152-157
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恩施地区粉葛不同组织活性成分含量与抑菌活性分析
(1.湖北民族大学生物科学与技术学院,湖北 恩施 445000;2.湖北省农业科学院中药材研究所,湖北 恩施 445000;3.国家中药材产业技术体系恩施综合试验站,湖北 恩施 445000)
Active Compound Content and Antibacterial Activity in Different Tissues of Pueraria thomsonii Benth from Enshi
(1.College of Biological Science and Technology,Hubei Minzu University,Enshi 445000,Hubei,China;2.Institute of Chinese Herbal Medicines,Hubei Academy of Agricultural Sciences,Enshi 445000,Hubei,China;3.National Traditional Chinese Medicine Industry Technology System Enshi Comprehensive Test Station,Enshi 445000,Hubei,China)
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投稿时间:2021-03-21    
中文摘要: 利用高效液相色谱法(high performance liquid chromatography,HPLC)测定粉葛不同组织的葛根素、鹰嘴豆芽素、大豆苷、大豆苷元的含量,并运用滤纸片法研究粉葛不同组织的抑菌效果。结果表明,大豆苷元、大豆苷2种黄酮活性成分在根中的含量最高,分别为(4.28±0.85)、(972.25±98.72)μg/g;葛根素在茎中的含量最高,为(7.37±1.14)mg/g;鹰嘴豆芽素在叶中的含量最高,为(33.48±4.01)μg/g。粉葛根部对金黄色葡萄球菌有中度敏感抑菌效果,其最低抑菌浓度(minimum inhibitory concentration,MIC)值为50.23 mg/mL;粉葛茎部对金黄色葡萄球菌、粪链球菌、苏云金芽孢杆菌、多耐铜绿假单胞菌有中度敏感抑菌效果,其MIC值分别为0.55、2.21、1.10、17.67 mg/mL;粉葛叶对苏云金芽孢杆菌有中度敏感抑菌效果,其MIC值为16.14 mg/mL。
中文关键词: 粉葛  葛根素  大豆苷  大豆苷元  鹰嘴豆芽素  抑菌
Abstract:The contents of puerarin,biochanin A,daidzin and daidzein in different tissues of Pueraria thomsonii were determined using high performance liquid chromatography.The antibacterial effect of different tissues of P.thomsonii was studied using the filter paper method.The results showed that the contents of daidzein and daidzin[(4.28±0.85)μg/g and(972.25±98.72)μg/g,respectively]were the highest in the root.Moreover,the content of puerarin[(7.37±1.14)mg/g]was the highest in the stem and that of biochanin A[(33.48±4.01)μg/g]was the highest in the leaves.The roots of P.thomsonii had a moderate sensitive bacteriostatic effect on Staphylococcus aureus,with a minimum inhibitory concentration(MIC)of 50.23 mg/mL.The stem of P.thomsonii had a moderate sensitive bacteriostatic effect on Staphylococcus aureus,Streptococcus faecalis,Bacillus thuringiensis,and pseudomonas aeruginosa,with MICs of 0.55,2.21,1.10,17.67 mg/mL respectively.The leaves of P.thomsonii had a moderate sensitive bacteriostatic effect on Bacillus thuringiensis,with an MIC of 16.14 mg/mL.
文章编号:202203023     中图分类号:    文献标志码:
基金项目:湖北省农业科学院青年科学基金项目(2022NKYJJ17);湖北省重点研发计划项目(2020BCA059);湖北省现代农业产业技术体系(HBHZD-ZB-2020-005)
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