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食品研究与开发:2022,43(3):94-101
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葡萄糖修饰苦杏仁粕谷蛋白糖基化反应条件的优化
(1.新疆农业大学食品科学与药学学院,新疆 乌鲁木齐 830052;2.新疆轻工职业技术学院,新疆 乌鲁木齐 830021)
Optimization of Conditions for Glycosylation of Glucose Modified Gluten in Bitter Apricot Kernel Meal
(1.College of Food Science and Pharmacy,Xinjiang Agricultural University,Urumqi 830052,Xinjiang,China;2.Xinjiang Industry Technical College,Urumqi 830021,Xinjiang,China)
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投稿时间:2021-02-23    
中文摘要: 为提高苦杏仁粕谷蛋白溶解性,采用糖基化方法改性修饰。该试验选用葡萄糖进行接枝改性,考察反应温度、反应时间、pH值、蛋白与糖质量比对葡萄糖改性蛋白溶解度的影响。在单因素试验的基础上,通过Box-Behnken响应面法确定葡萄糖改性蛋白的制备工艺。结果表明,优化后工艺条件为反应温度63℃、反应时间0.6 h、pH 9.4、蛋白与糖质量比1∶2。在此条件下,葡萄糖改性蛋白溶解度为96.24%。
中文关键词: 苦杏仁粕  谷蛋白  葡萄糖  糖基化处理  工艺
Abstract:To improve solubility of gluten from bitter apricot kernel meal,a method was developed to glycosylate gluten.The effects of reaction temperature,reaction time,pH and protein-sugar mass ratio on the solubility of glucose modified protein were investigated.Box-Behnken response surface methodology based on single factor testing was used to optimize a preparation process for glucose modified protein.The optimal process conditions were as follows:reaction temperature 63 ℃,reaction time 0.6 h,pH 9.4,protein-sugar mass ratio 1∶2.Under these conditions,glucose modified protein solubility was 96.24%.
文章编号:202203014     中图分类号:    文献标志码:
基金项目:国家重点研发计划项目(2019YFD1002300)
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