Abstract:This study was conducted to explore whether probiotic and prebiotic combination could improve the antibiotic-induced dysbiosis of intestinal microbiota in juvenile mice.A total of 45 4-week-old male Balb/c mice were randomly divided into the control，antibiotic，and treatment groups （n=15/group）.The tested mice in the treatment group were orally administered ceftriaxone and the combination of probiotics and prebiotics every day for one week.Meanwhile，the mice in the antibiotic and control groups were administered ceftriaxone and normal saline，respectively，every day for one week.It was concluded that ceftriaxone can significantly damage the normal structure of intestinal microbiota and metabolic function of short-chain fatty acids in juvenile mice，while no significant negative effects on the growth and immune system of juvenile mice were found.Probiotic and prebiotic combination might be beneficial to restore the metabolism of short-chain fatty acids and modulate the immune function，while it showed limited effects on intestinal microbiota dysbiosis induced by antibiotics.