Abstract:This study aimed to characterize the effects of Kuding Cha on body weight，liver，kidney，and oxidative stress in mice fed a high choline diet.The active components of Kuding Cha were analyzed by ultra performance liquid chromatography-time of flight-mass spectrometry.C57BL/6J mice were randomly divided into a blank control group，a model control group，and low，medium，and high dose Kuding Cha groups.Food intake and body weights were monitored，and blood，liver，and kidney samples were collected for analyses after 4 months of intervention.The diet and excretion of mice in each group were normal，compared with the model control group，body weight gain in each dose group was decreased，total protein content increased in the high dose group，malondialdehyde levels decreased in each dose group，and superoxide dismutase activity increased significantly in the medium dose group.There was no significant difference in organ coefficient，and no obvious chronic or acute liver or kidney injury.A high choline diet could cause abnormal growth rates，increase malondialdehyde levels，and decrease superoxide dismutase activity in mice.The appropriate dose of aqueous Kuding Cha extract could reduce symptoms of weight and oxidative damage in mice.