Abstract:A method for the determination of taurine content in eggs by high performance liquid chromatography（HPLC）was established，and the taurine contents in egg yolks of dark eggs（brands A and B），native eggs（brands C and D），and ordinary eggs was analyzed and compared.In the experiment，ortho-phthalaldehyde was used as the derivating agent，and the on-line derivatization function of the auto sampler was used for precolumn derivatization.A methanol/acetonitrile:water （25∶75，volume ratio）mobile phase and fluorescence detection were used.The results demonstrated good linearity in the concentration range of 1 μg/mL to 25 μg/mL（R2=0.999 9）.The limit of detection was 0.01 μg/mL，the recovery range was 99.7% to 100.5%，and the relative standard deviation （RSD）range was 0.5% to 0.6%.The taurine content of brands A and B （dark eggs），brands C and D （native eggs），and ordinary eggs was 1.332，1.151，1.063，0.735，0.622 mg/100 g，respectively.The taurine content in the yolk of ordinary eggs was significantly lower than that in brands A and B（dark eggs）and brand C（native eggs，P＜0.05），and slightly lower than that in brand D（native eggs，P＞0.05）.The method was simple，accurate，specific，and suitable for the determination of taurine content in eggs.