Abstract:The dried flower buds of Paeonia lactiflora Pall.were used as the raw materials to prepare a beverage with extract from flowers.Considering the comprehensive efficacy of several activities，including in vitro antioxidation，tyrosinase inhibition and pancrelipase inhibition as the indicators，the extraction from Paeonia lactiflora Pall.flowers was optimized based on a single factor experiment and an orthogonal test.The results revealed that the best extraction method was microwave-assisted extraction and optimal extraction conditions were confirmed as follows：the extraction time of 2 min，microwave power of 480 W，solid-to-liquid ratio of 1∶20（g/mL），ethanol concentration of 60% and the number of extraction as 3 times.Then，the additives were added into the extract，and sensory evaluation was used as an indicator to optimize the flavor of the extracted beverage based on the orthogonal test.The optimized formula of the extracted beverage was confirmed as follows：the concentration of aspartame，citric acid and ethyl maltol was 0.032%，0.4% and 0.06% respectively.The comprehensive score of the prepared beverage was considerably high，with bright color and unique flavor.