Abstract:Chalcone isomerase（CHI），a key enzyme in the biosynthesis of plant flavonoids.To further study the structure and function of chalcone isomerase，bioinformatics methods were used to analyze the pomegranate CHI gene.Results showed that pomegranate CHI was a hydrophilic protein that was acidic，with no transmembrane domain or signal peptide，subcellularly localized in the mitochondria，and had a phosphorylation site，albeit without a glycosylation site.The amino acid sequence had a chalcone 3 chalcone superfamily conservative domain.Furthermore，α-helix and random coil were the main secondary structure components.Additionally，the homology modeling method was used to construct a three-level structure model.Results show that the model was consistent with the secondary structure prediction results，with a better model quality evaluation result.