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投稿时间：2021-03-25
投稿时间：2021-03-25
中文摘要: 该试验研究复合酶协同超高压法提取黑木耳多糖最佳工艺条件。以黑木耳多糖得率为指标，采用单因素试验和正交试验，确定最佳提取工艺参数。结果表明，复合酶提取最佳工艺参数为酶解时间50 min，复合酶（纤维素酶∶木瓜蛋白酶=1∶1，质量比）添加量3%，酶解温度50℃，酶解pH值6.5。在此条件下，黑木耳多糖得率为9.26%。经复合酶法优化后，再进行超高压法提取，最佳工艺参数为保压时间8 min，提取温度50℃，压力400 MPa，料液比1∶30（g/mL）。在此条件下，黑木耳多糖得率为12.23%。
Abstract:In this study，the optimum conditions for the extraction of polysaccharides from Auricularia auricular by compound enzyme-assisted ultra-high pressure were studied.The yield of A.auricular polysaccharides was used as the index，and the optimal process parameters were determined by single-factor and orthogonal tests.The optimum process parameters for compound enzyme extraction were found to be enzymolysis time of 50 min，compound enzyme addition （1∶1 cellulase:papain，mass ratio）at 3%，enzymolysis temperature of 50 ℃，and enzymolysis pH of 6.5.Under these conditions，the yield of A.auricular polysaccharide was 9.26%.Ultra-high pressure extraction was carried out after the optimization of the compound enzyme method.The optimum process parameters for ultra-high pressure extraction were holding time of 8 min，extraction temperature of 50 ℃，pressure of 400 MPa，and solid∶liquid ratio of 1∶30（g/mL）.Under these conditions，the yield of A.auricular polysaccharides was 12.23%.
keywords: Auricularia auricular polysaccharides ultra high pressure compound enzyme process optimization
文章编号：202124016 中图分类号： 文献标志码：
基金项目:
| 作者 | 单位 |
| 隋志方，刘延奇，秦令祥 | 鹤壁市质量技术监督检验测试中心，河南 鹤壁 458030；郑州轻工业大学食品与生物工程学院，河南 郑州 450002；漯河市食品研究院有限公司，河南 漯河 462300 |
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