Abstract:Degumming is one of the key processes in oil refining.This study opted to use the ultrasonic coenzyme method to study the optimal conditions in which to degum sea buckthorn fruit oil.Phospholipase A1（phospholipase A1，PLA1)was selected to be used in this study.The effects that the changes to certain factors had on the sea buckthorn fruit oil，were studied until a full set of optimal conditions for the degumming process could be derived.The factors that were examined included the quantity of enzyme added ultrasonic time used，ultrasonic temperature applied，the pH value of the oil,amount of water added,and ultrasonic frequencies that were applied during the degumming process.The response surface method was used to optimize the conditions of the degumming process；this was selected using the single factor test along with the box-Behnken central combined testing design principle.According to the results，the optimal conditions were as follows：an enzyme dosage of 5%，ultrasonic time of 39 minutes，ultrasonic temperature of 50℃，and an ultrasonic power of 180 W.Under these conditions，the predicted phosphorus content of sea buckthorn fruit oil was 0.095 mg/g.The measured value was 0.097 mg/g，with an error rate of 2.100%.The low error rate proved that the derived model was reliable and could be used for optimal degumming of sea buckthorn fruit oil.