Abstract:This study analyzed and compared the current situation of two pre-treatment technologies of chemical（3-aminobenzoic acid ethyl ester methanesulfonate，eugenol，CO2，etc.）and physical（cooling，electric shock，etc.）methods for fish.Two forms of keep-alive transportation:water and waterless transportation were used in this study，and stress responses（oxidative stress，immune responses，and energy metabolism）of fish during transportation were briefly described.Current research progresses on keep-alive transportation technology of fish were summarized，in order to provide reference for future research.