Abstract:The extraction solvent and material-liquid ratio were selected to optimize the essential oil extraction process of Citrus reticulata Blanco cv.Manau Gan.Results showed that the most suitable solvent for the essential oil extraction of Citrus reticulata Blanco cv.Manau Gan was petroleum ether，with a material-to-liquid ratio of 1∶15（g/mL）.Three factors—ultrasonic time，temperature，and power—were selected for a single-factor experiment；an orthogonal condition optimization experiment was carried out for the ultrasonic extraction parameters of the essential oil of Citrus reticulata Blanco cv.Manau Gan.The optimization results were as follows：ultrasound time，120 min；ultrasound temperature，50 ℃；and ultrasonic power，60 W.The essential oil extraction rate of Citrus reticulata Blanco cv.Manau Gan reached 2.47%under these conditions.A total of 19 compounds were identified from the essential oil of Citrus reticulata Blanco cv.Manau Gan by gas chromatography-mass spectrometry analyses，accounting for 97.60%of total volatiles.Thus，the optimized process could be used to extract the essential oil of Citrus reticulata Blanco cv.Manau Gan.