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投稿时间：2021-06-03
投稿时间：2021-06-03
中文摘要: 建立快速高效检测乳制品中牛磺酸含量的程序进样-柱前衍生-高效液相色谱法。该方法对牛磺酸提取试剂、衍生化试剂、衍生方式、仪器色谱条件进行优化。通过设定进样程序，简化样品前处理衍生操作，大幅度缩短了检测时间。试验结果表明，方法加标回收率为99.2%～104.0%，相对标准偏差（relative standard deviation，RSD）为3.22%～5.25%，线性相关系数R2=0.999 9，定量限为0.2 mg/100 g。同时对牛乳羊乳中的牛磺酸含量进行测定，结果显示全脂牛奶粉和全脂羊奶粉中牛磺酸的含量范围分别为2.89mg/100g～7.42mg/100g和36.6 mg/100 g～60.4 mg/100 g；纯牛奶和纯羊奶中牛磺酸的含量范围分别为0.37mg/100 g～0.68 mg/100 g和4.20 mg/100 g～6.10 mg/100 g。羊乳的牛磺酸均明显高于牛乳，其含量比值≥9，差异显著。
Abstract:A rapid and efficient method for the determination of taurine content in dairy products was established.Extraction reagent，derivatization reagent，derivatization method and instrumental chromatographic conditions for evaluation of taurine content were optimized.By setting the injection procedure，sample pretreatment and derivatization operation were simplified，and detection time was greatly shortened.The results showed that recoveries were 99.2%-104.0%，relative standard deviation was 3.22%-5.25%，linear correlation coefficient R2 was 0.999 9，and limit of quantification was 0.2 mg/100 g.In addition，taurine content in whole cow milk powder/whole goat milk powder was 2.89 mg/100 g-7.42 mg/100 g and 36.6 mg/100 g-60.4 mg/100 g，respectively，while that in pure cow milk/pure goat milk was 0.37 mg/100 g-0.68 mg/100 g and 4.20 mg/100 g-6.10 mg/100 g，respectively.Taurine content in goat milk was significantly higher than that in cow milk，and its content ratio was more than or equal to 9.
keywords: program sampling precolumn derivatization high performance liquid chromatography taurine dairy products
文章编号：202121018 中图分类号： 文献标志码：
基金项目:国家市场监督管理总局科技计划项目（2019MK062）
| Author Name | Affiliation |
| WU Hai-zhi，XIAO Yue-hui-zi，ZHU Li，YUAN Lie-jiang，WANG Shu-xia，HUANG Yuan-yuan | Hunan Testing Institute of Product and Commodity Supervision，Changsha 410007，Hunan，China |
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