Abstract:In the study，the most significant factors influencing quinoa flavonoid yields were examined；these included ultrasonic power，ultrasonic immersion time，material ratio，and ethanol concentration.The levels of these various factors were determined using a single-factor experiment，with the results of the orthogonal test as a reference，and the best process conditions for extracting quinoa flavonoids were determined.Perforation and double dilution，which were used to research antiseptic effects，were adopted to analyze the effect of Staphylococcus aureus.The results showed that，the material ratio reached 1∶52（g/mL），with an ultrasonic power of 235 W，ethanol concentration of 78%，and ultrasonic extraction time of 26 min.0.280% was the highest quinoa flavonoid yield rate in these conditions.The ethanol extract of quinoa flavonoids positively affected the bacteriostatic effect，and the bacteriostatic effect increased with the increase of flavonoid concentration.The minimum inhibitory concentration（MIC）of quinoa flavonoids was 7.500 mg/mL with the double dilution method.