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投稿时间:2020-08-11
投稿时间:2020-08-11
中文摘要: 研究蛋黄果的果肉多酚和种子多酚的提取以及其抗氧化活性将为蛋黄果的综合开发利用提供理论基础。在分析超声波辅助法提取果肉多酚和种子多酚单因素影响的基础上,运用响应面法优化多酚的提取工艺从而获得果肉多酚的最佳提取条件为乙醇浓度50%、提取时间102 min、提取温度62℃、料液比1∶30(g/mL);种子多酚的最佳提取条件为乙醇浓度40%、提取时间101 min、提取温度60℃、料液比1∶30(g/mL),此时果肉多酚和种子多酚提取率分别为0.74%、2.65%,与预测值(0.71%、2.69%)相接近。对1,1-二苯基-2-三硝基苯肼自由基(DPPH·)、羟自由基(·OH)、超氧阴离子自由基(O2-·)的清除能力试验表明果肉多酚和种子多酚都具有较强抗氧化性,并与浓度呈正相关,且种子多酚的抗氧化活性高于果肉多酚,说明蛋黄果种子可以作为天然抗氧化剂的来源。
Abstract:Polyphenols were extracted from the pulp and seeds of Pouteria campechiana,and their antioxidant activities were analyzed,providing a theoretical basis for the development and utilization of P.campechiana.Ultrasound-assisted extraction was used to extract polyphenols from the pulp and seeds.Based on single-factor experiments,the response surface methodology was used to optimize the extraction conditions.The optimal extraction conditions for pulp polyphenols were:ethanol concentration 50%,extraction time 102 min,extraction temperature 62 ℃,ratio of material to water 1∶30(g/mL).The optimal extraction conditions for seeds polyphenols were:ethanol concentration 40%,extraction time 101 min,extraction temperature 60 ℃,and material-towater ratio 1∶30(g/mL).Under these conditions,the polyphenol extraction yields from the pulp and seeds were 0.74% and 2.65%,respectively,which were close to the predicted values(0.71% and 2.69%,respectively).The antioxidant capacity of the pulp and seed polyphenols was evaluated by determining the scavenging capacities of DPPH·,·OH,and O2-·.The results showed that both pulp and seed polyphenols exhibited a strong antioxidant capacity,which was positively correlated with the concentration,and that the antioxidant capacity of the seeds polyphenol was higher than that of the pulp polyphenol.The results indicated that P.campechiana could be utilized as a natural source of antioxidant components.
文章编号:202113021 中图分类号: 文献标志码:
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