Abstract:In order to efficiently extract the collagen from yak tendon，the yak tendon was used as raw material to extract the collagen by hot water and enzymolysis method，and the best extraction method was selected.On the basis of the optimal extraction method，the effects of different ratios of solid-to-liquid ，temperature，time and enzyme dosage on the yield，extraction rate and purity of yak tendon collagen were studied by single factor experiment.The extraction conditions were optimized by orthogonal experiment.The results showed that enzymolysis method had better effect on yak tendon collagen extraction.The optimal extractionconditionswere determineas follows:solid-to-liquid ratio 1∶12 （g/mL），and enzyme dosage 2.5%，and extraction at 43 ℃ for 11 h.Under these conditions，the yield，extraction rate and purity of collagen were 14.91%，69.94% and 89.44%，respectively.