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投稿时间：2020-10-13
投稿时间：2020-10-13
中文摘要: 采用超声辅助水提法制备橄榄叶提取物（olive leaf extracts，OLE），测定其多酚含量，并研究OLE对辐射小鼠的防护作用。给予小鼠不同剂量OLE（150、500、1 000 mg/kg）后，1 Gy X射线全身辐照小鼠，测定辐射小鼠外周血细胞数量、血清超氧化物歧化酶（superoxide dismutase，SOD）与谷胱甘肽过氧化物酶（glutathione peroxidase，GSH-Px）活力、丙二醛（malondialdehyde，MDA）含量、骨髓细胞微核率和肝脏Bcl-2与Bax凋亡蛋白的表达水平。结果表明，OLE中的多酚含量丰富，高达（57.55±2.98）mg/g，可有效提高小鼠外周血细胞数量与血清SOD、GSH-Px活性，降低MDA含量，抑制骨髓细胞微核的形成，调控肝脏凋亡蛋白的表达，对辐射损伤具有良好的防护作用。
Abstract:Olive leaf extract（OLE）was extracted by using ultrasonic-assisted water extraction method and its polyphenol content was determined.Its protective effect on the damage of mice induced by radiation was investigated as well.Different doses of OLE （150，500，1 000 mg/kg）were given to mice before they were irradiated at the dose of 1 Gy.The number of peripheral blood cells，the activities of superoxide dismutase（SOD），glutathione peroxidase（GSH-Px），and the content of malondialdehyde（MDA）of mice were measured.The bone marrow micronucleus cells and the expression of apoptosis related proteins of liver were detected.The results showed that olive leaves were rich in polyphenols and the content was as high as（57.55±2.98）mg/g.OLE could effectively increase the number of peripheral blood cells and the activities of SOD and GSH-Px in serum，reduce the content of MDA，inhibit the formation of micronucleus in bone marrow cells and regulate the expression of apoptotic proteins.It exhibited protective effect on radiation-induced damage.
keywords: olive leaf polyphenols radiation damage peripheral blood cells oxidative damage micronucleus
文章编号：202106009 中图分类号： 文献标志码：
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