Abstract:In order to explore the utilization value of economic fish guts，the crude fatty acids of five kinds of economic fish guts were extracted by hydrolysis method，and the contents and composition of fatty acids were analyzed via external standard method of gas chromatography.The results showed that a total of 30 fatty acids were found in 5 types of economic fish guts，including 11 saturated fatty acids，8 monounsaturated fatty acids and 11 polyunsaturated fatty acids.The content of total fatty acids in Cololabis saira was the highest（2 504.27±64.99）mg/100 g and that in Aristichthys nobilis was the lowest （712.28±5.82）mg/100 g.The proportion of unsaturated fatty acids was between 56.78% and 85.06%，except that the proportion of monounsaturated fatty acids was 31.37% in Cololabis saira.The contents of essential fatty acids of the three freshwater fish guts were higher（23.75%-40.85%），but the contents of eicosapentaenoic acid（EPA）and docosahexenoic acid（DHA）were lower.In the seawater fish guts，the proportion of EPA+DHA contents in the Pneumatophorus japonicus gut was higher（30.88%），but the content of essential fatty acids was lower（less than 6%）.The above results showed that the fatty acids of some economic fish guts were rich，among which the essential fatty acids of freshwater fish were higher，and the DHA content of seawater fish was very high，which had the value of further development and utilization.