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投稿时间：2020-09-14
投稿时间：2020-09-14
中文摘要: 该文以出芽短梗霉（Aureobasidium pullulans）为生产菌株，通过单因素和响应面试验对培养基组分进行优化以提高黑色素产量。结果表明，出芽短梗霉发酵产黑色素的最佳培养基组成为：蔗糖98.9 g/L、玉米浆干粉9.7 g/L、CaCl2·2H2O 2.0 g/L、NaCl 2.0 g/L、ZnSO41.0 g/L、pH 5.5。在此条件下，经过 144 h 发酵，黑色素产量达到 17.56 g/L，相比优化之前提高了42.8%。
Abstract:Based on a strain of Aureobasidium pullulans，an optimization on medium components was performed by means of single-factor experiment and response surface method to improve melanin yield.The results showed that the optimum medium components were as below that sucrose98.9 g/L，corn steep flour 9.7 g/L，CaCl2·2H2O 2.0 g/L，NaCl 2.0 g/L，ZnSO41.0 g/L，pH 5.5.Under above described conditions，a desirable melanin yield of 17.56 g/L was achieved after 144 h fermentation which was 42.8 % up than that before optimization.
keywords: Aureobasidium pullulans melanin fermentation technology optimization of culture medium response surface method
文章编号：202103028 中图分类号： 文献标志码：
基金项目:天津市科技计划项目（17YFCZZC00310）
| 作者 | 单位 |
| 魏永昌，袁凯凯，李振海，仝雅娜，乔长晟 | 天津科技大学生物工程学院，天津 300457；天津市工业微生物重点实验室，工业发酵微生物教育部重点实验室，天津 300457；天津北洋百川生物技术有限公司，天津 300457；天津市设施农业研究所，天津 300112 |
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