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投稿时间：2020-10-15
投稿时间：2020-10-15
中文摘要: 通过优化有机溶剂种类和光化学衍生时间，建立采用光化学衍生进行前处理，并利用黄曲霉毒素原位衍生荧光检测仪快速检测黄曲霉毒素荧光强度的方法。结果表明，在光化学衍生时间为4 min时，70%甲醇溶剂中黄曲霉毒素B1和G1的光化学衍生荧光增强效果最显著，优于甲醇和乙腈；黄曲霉毒素B2和G2荧光强度最大且稳定，光化学衍生后无明显增强。该方法在0.1 ng/mL～20.0 ng/mL线性关系良好，R2＞0.998 4，方法检测限在0.15 ng/mL～0.37 ng/mL之间，检测时间短，操作简单方便，满足食品和中药材中黄曲霉毒素的快速检测要求。
Abstract:Through the optimization of organic solvent type and photochemical derivatization time，a method for rapid detection of aflatoxin fluorescence intensity was established by using photochemical derivatization pretreatment and aflatoxin insitu derivatization fluorescence detector.The results showed that the fluorescence enhancement effect of aflatoxin B1and G1in 70 % methanol solvent was the most significant when the time of photochemical derivatization was 4 min，which was better than that of methanol and acetonitrile.At the same time，the fluorescence intensity of aflatoxin B2and G2in 70 % methanol solvent was the highest and stable，but there was no obvious enhancement after photochemical derivatization.The method had a good linear range when the concentration of aflatoxin was between 0.1 ng/mL-20.0 ng/mL，R2＞0.998 4，the limit of detection was between 0.15 ng/mL and 0.37 ng/mL，with short detection time，simple and convenient operation，and it could meet the requirements of rapid detection of aflatoxin in food and Chinese medicine materials.
keywords: aflatoxin insitu photochemical derivatization fluorescence intensity rapid detection food and Chinese medicinal materials
文章编号：202103025 中图分类号： 文献标志码：
基金项目:广东省科学院发展专项（2019GDASYL-0105024）
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