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投稿时间：2020-06-28
投稿时间：2020-06-28
中文摘要: 结合间接竞争反应机制，运用酶联免疫吸附技术（enzyme-linked immunosorbent assay，ELISA），从抗原（antigen，Ag）包被时间、体系反应温度、酶标二抗作用时间、显色时间等主要因素开展快速检测黄曲霉毒素改进方法的研究。研究结果表明适宜的优化条件为：采用Ag包被20 h、反应温度24℃左右、酶标二抗作用时间30 min、底物显色时间15min。通过对饲料等11种样本的检测得出：半抑制浓度IC50＜0.1μg/L，添加回收率在67%～116%，灵敏度达到0.03 μg/L，线性系数＞0.99，板内变异小于5%。检测试验结果良好，表明该改进方法具有可行性。
Abstract:Combined with indirect competitive response mechanism，enzyme-linked immunosorbent assay（ELISA）was used to study improved methods for rapid detection of aflatoxin from main factors of antigen（Ag）including duration of inclusion，systemic reaction temperature，time of enzyme-linked immunosorbent assay，color development time，etc.The results showed that the optimal conditions were as follows：Ag coating was used for 20 h，the reaction temperature was around 24℃，the reaction time was 30 min，and the substrate chromogenic time was 15 min.Through the detection of 11 kinds of samples，such as feed，it could be concluded that the semi-inhibitory concentration IC50was less than 0.1 μg/L，the recovery rate was 67 %-116 %，the sensitivity and effective detection were 0.03 μg/L，the linear coefficient was greater than 0.99，and the in-board variation was less than 5 %.The good test results showed that the improved method was feasible.
keywords: enzyme-linked immunosorbent assay（ELISA） aflatoxins analysis and determination experimental optimization accuracy
文章编号：202103024 中图分类号： 文献标志码：
基金项目:天津市科技计划项目（14ZCZDNC00003）
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