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食品研究与开发:2021,42(3):31-36
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菌酶协同处理优化亚麻籽粕风味的工艺研究
(1.天津科技大学食品科学与工程学院,天津 300457;2.天津市利民调料有限公司,天津 300000;3.军事科学院系统工程研究院军需工程技术研究所,北京 100010)
Optimization of Preparation Technology of Flavor of Flaxseed Meal Produced by Fermentation with Enzyme Addition
(1.College of Food Science and Engineering,Tianjin University of Science and Technology,Tianjin 300457,China;2.Tianjin Limin Condiment Co.,Ltd.,Tianjin 300000,China;3.Institute of Quartermaster Engineering and Technology,Institute of System Engineering,Academy of Military Sciences,Beijing 100010,China)
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投稿时间:2020-08-31    
中文摘要: 亚麻籽粕风味独特,该文采用固态发酵酶解法增强亚麻籽粕的风味,探讨风味强化的最佳工艺。以氨基态氮和游离脂肪酸(free fatty acid,FFA)含量为指标,并对亚麻籽粕、发酵产物、发酵酶解液进行感官评价。结果表明,黑曲霉发酵亚麻籽粕的工艺条件为:料水比1∶1.0(g/mL),黑曲霉添加量6%,葡萄糖添加量6%,37℃发酵5 d,此时发酵产物中氨基态氮含量为1.92 g/100 g,游离脂肪酸(FFA)含量为0.56%;选取发酵底物并配制成5%的发酵液进行中性蛋白酶酶解,酶解最佳条件是加酶量2%,55℃酶解2 h,此时酶解液的氨基态氮含量为4.03 g/100 g,游离脂肪酸(FFA)含量是22.10%;与未发酵和酶解的亚麻籽粕相比,氨基氮含量增加4.03 g/100 g,游离脂肪酸(FFA)含量扩大246倍;感官分析表明,亚麻籽粕发酵酶解液香气浓郁、无异味,色泽均匀一致,组织状态细腻、均匀。
Abstract:Flaxseed meal has a unique flavor.In this paper,the solid-state fermentation enzymatic hydrolysis was used to enhance the flavor of flaxseed meal,and process optimization was discussed.Amino nitrogen content and free fatty acid content(free fatty acid,FFA)were used as indicators.The sensory evaluation of flaxseed meal,fermentation products and enzymatic hydrolysate was carried out.The results demonstrated that optimized conditions (material-liquid ratio,Aspergillus niger content,glucose content,fermentation temperature,fermentation time)for fermented flaxseed meal were 1∶1.0(g/mL),6 %,6 %,37℃ and 5 d,respectively.Statistical analysis showed that under optimal fermentation conditions,the amino nitrogen content and free fatty acid content were 1.92 g/100 g and 0.56 %,respectively.Then,using 5 % fermentation liquid of flaxseed meal as raw materials,neutral protease was used for enzymolysis.The results showed that the optimal enzymatic hydrolysis process was as follows:the addition of neutral protease was 2 %,the enzymatic hydrolysis time was 2 h and enzymatic hydrolysis temperature was 55℃.The yield of the amino nitrogen content was 4.03 g/100 g and free fatty acid content was 22.10 %.After such enzymatic hydrolysis with protease,the amino nitrogen content and free fatty acid content increased significantly(by 4.03 g/100 g and 246 times respectively).Sensory evaluation showed good smell,appearance,taste of enzymatic hydrolysate of flaxseed meal fermentation.
文章编号:202103006     中图分类号:    文献标志码:
基金项目:天津市自然科学基金项目(18JCYBJC43700);天津市一二三产业融合发展科技示范工程(18ZXYENC00090);天津市合成生物技术创新能力提升行动重大科技攻关任务(TSBICIP-KJGG-004-09)
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