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投稿时间：2020-08-01
投稿时间：2020-08-01
中文摘要: 为了建立牛组织中常山酮残留量的超高效液相色谱-串联质谱检测方法，将牛组织中的常山酮经蛋白酶酶解后，采用乙酸乙酯提取，HLB固相萃取柱净化，超高效液相色谱-串联质谱法进行检测。在0.5 ng/mL～50 ng/mL线性范围内，常山酮的回归方程呈良好的线性关系，R2＞0.998，牛组织样品在添加水平为0.5、5 μg/kg时，回收率为73.2%～93.60%，相对标准偏差（relative standard deviation，RSD）为 5.91%～7.96%，方法检出限为 0.25 μg/kg，定量限为 0.5 μg/kg。本试验适用于牛组织中常山酮残留量的检测。
中文关键词: 超高效液相色谱-串联质谱法 牛组织 常山酮 检测 固相萃取
Abstract:To develop a method for determination of halofuginone in bovine tissues by ultra performance liquid chromatography-tandem mass spectrometry（UPLC-MS/MS）.Bovine tissues samples were enzymolysed with trypsin，and extracted with ethyl acetate，then cleaned up with SPE column.Finally，the analytes were tested by UPLC-MS/MS.The matrix-matched calibration curve of halofuginone showed a good linear within the concentration range from 0.5 ng/mL to 50 ng/mL （R2＞0.998）.In the bovine tissues samples，the recovery of halofuginone at the spiked levels of 0.5，5 μg/kg was from 73.2% to 93.60%，and the relative standard deviation（RSD）was between 5.91% and 7.96%，and the limit of detection was 0.25 μg/kg，the limit of quantitation was 0.5 μg/kg.The method is suitable for the detection of halofuginone in bovine tissues.
keywords: ultra performance liquid chromatography-tandem mass spectrometry（UPLC-MS/MS） bovine tissue halofuginone determination solid-phase extraction
文章编号：202102028 中图分类号： 文献标志码：
基金项目:
| Author Name | Affiliation |
| LI Ya-qiong，XIE Peng | Luohe Vocational College of Food，Luohe 462300，Henan，China |
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