Abstract:The effects of different doses of inulin on the anti-fatigue effect of mice and the effects on intestinal flora were studied.After adding different doses（0%，5%，10% and 20%）of inulin to the mice's feed and 120 days of feeding，the weight and swimming time of the mice were measured，and the serum was tested for creatine kinase（CK）activity，blood lactic acid（BLA）and blood urea nitrogen（BUN）content and mouse fecal flora structure.Results showed that，compared with the blank control group，the mice in the treatment group prolonged the weight-bearing swimming time（p<0.05），and the increase in the middle-dose group was greater than that in the other treatment groups；with the increase of the dose，the CK activity，BLA and BUN content showed A similar downward trend，and the medium and high dose treatment groups were significantly different from the blank group （p<0.05）；with the increase of inulin dose，the content of bifidobacteria in the intestine showed an increasing trend，but appeared under high dose treatment a large number of potential pathogenic bacteria multiply.In conclusion，inulin increased the duration of weight-bearing swimming.Meanwhile，the increase of CK activity，BLA and BUN contents caused by strenuous exercise were inhibited.Inulin showing a good anti-fatigue effect.In addition，inulin had an improved effect on the diversity of intestinal flora，among which 10% inulin was the best dose for anti-fatigue effect and improvement of intestinal microbial flora in mice.