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食品研究与开发:2020,41(21):103-108
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蟛蜞菊总黄酮的超声波辅助提取工艺优化及其抗氧化能力的研究
(广西民族师范学院生物与食品工程学院广西高校桂西南特色植物资源化学重点实验室培育基地,广西崇左532200)
Study on the Optimization of Ultrasonic-assisted Extraction Technology and Antioxidant Capacity of Total Flavonoids from Wedelia chinensis
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投稿时间:2019-11-26    
中文摘要: 以蟛蜞菊为研究对象,采用超声波辅助提取蟛蜞菊总黄酮,确定蟛蜞菊总黄酮的最佳提取工艺条件,并对其抗氧化能力进行探究。结果显示,蟛蜞菊总黄酮的最佳提取条件为:乙醇浓度45%,料液比1∶30(g/mL),超声功率40 W,提取温度60℃,提取时间40 min,此条件下,蟛蜞菊总黄酮的得率高达6.64%。蟛蜞菊总黄酮的抗氧化能力结果表明,蟛蜞菊总黄酮提取液的质量浓度越大,对·OH、DPPH·以及NaNO2的清除能力就越强。当蟛蜞菊总黄酮的质量浓度为0.664 mg/mL时,对DPPH·、·OH、NaNO2的清除率分别为94.62%、76.29%、63.99%,即DPPH·的清除率>·OH的清除率>NaNO2的清除率。由此可见,蟛蜞菊总黄酮具有较好的抗氧化活性。
Abstract:The total flavonoids of Wedelia chinensis were extracted by ultrasonic method,and the optimal extraction conditions were determined,and the antioxidant capacity was explored.It was found that the best extraction conditions of total flavonoids were:ethanol concentration 45%,material liquid ratio 1 ∶30(g/mL),ultrasonic power 40 W,extraction temperature 60℃,extraction time 40 min.Under these conditions,the yield of total flavonoids was as high as 6.64%.The results showed that the higher the concentration of total flavonoids,the stronger the scavenging ability to·OH,DPPH· and NaNO2.When the total flavone concentration of Wedelia chinensis was 0.664 mg/mL,the scavenging rate of DPPH·,·OH and NaNO2were 94.62%,76.29% and 63.99%,respectively,that was,the scavenging rate of DPPH·>·OH > NaNO2.It could be seen that the total flavonoids of Wedelia chinensis had good antioxidant activity.
文章编号:202021018     中图分类号:    文献标志码:
基金项目:2011年度“广西高校优秀人才资助计划”项目(桂教人[2011]40号);广西民族师范学院学科专业带头人科研启动项目(2014RCDT001)
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