Abstract:The traditional method of multi-pesticide residue detection is tedious and time-consuming.At present，the detection methods of multi-pesticide residues are developing towards automation，fast，new and simple.In order to meet the needs of multi-pesticide residues detection，many chemometric methods have been proposed for rapid and automated analysis.However，there are still many problems with many parameters and low accuracy.In this paper，the moving window target transfer factor method was improved to achieve automated analysis of multi-pesticide residue.240 pesticide standard mass spectra database was established.The improved moving window target transfer factor method was used to the automatically analysis of the unknown pesticide mixed standard，and the results under standard elution and fast elution conditions were compared.The results showed that the improved moving window target transfer factor analysis method could be used to the automate identification of multi-pesticide residues，and the detection results obtained under the fast elution conditions were basically the same as those obtained with the standard elution conditions，indicating that the improved moving window target transfer factor was not affected by elution conditions.The method provides a reference for the automated detection of samples of multi-pesticides in samples.