Abstract:A total of 41 endophytic fungi strains were isolated from the healthy tissue（stem，leaf，and fruit）of the"pink blue"blueberry from Majiang blueberry cultivation base in Guizhou，which were categorized into 8 morphotypes according to culture characteristics，and the dominant genus was Passalora sp.；confrontation test with 5 pathogens showed that the growth of all 5 disease strains were inhibited by endophytic fungi BB01，BL22，BF27，BF40 and BF41；mycelial growth test was performed using secondary metabolites crude extracts of these endophytic fungi，when the concentration of BB01，BL22，BF27，BF40 and BF41 crude extracts was 500 μg/mL，the growth of pathogens were inhibited over 90%，indicating significant anti-fungal activity；rDNA-ITS sequences were amplified by PCR for phylogenetic tree construction，the results showed that BF40 was identified as Colletotrichum sp.，BF40 was identified as Pallidocercospora sp.and BL22，BF27 and BB01 were identified as Cladosporium sp..