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食品研究与开发:2020,41(19):25-29
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不同水解度对核桃分离蛋白酶解物功能特性的影响
(1.吕梁学院生命科学系,山西吕梁033000;2.山西省特色植物功能成分工程研究中心,山西吕梁033000)
Effects of Different Degree of Hydrolysis on Functional Characteristics of Enzymatic Hydrolysate of Walnut Protein
(1.Department of Life Science,Lvliang University,Lvliang 033000,Shanxi,China;2.Shanxi Engineering Research Center of Plant Functional Composition,Lvliang 033000,Shanxi,China)
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投稿时间:2020-04-12    
中文摘要: 核桃中富含脂肪、蛋白质和健康的纤维,其应用研究已引起广泛关注。该文以核桃饼粕为原料,通过酸沉碱提法分离出蛋白,以碱性蛋白酶制备不同酶解程度的核桃蛋白。对其持水性、乳化性和乳化稳定性、起泡性和泡沫稳定性进行测定。结果表明,随着酶解时间的增长,水解度在120 min达到最大为19.4%;持水性和乳化性随酶解程度的增大呈现先上升后下降的趋势,起泡性和乳化稳定性随酶解程度的增大而增大,泡沫稳定性随酶解程度增大而减小。这说明水解度可以提高也可削弱核桃分离蛋白酶解物的某些功能特性。该研究结果为深入利用核桃饼粕奠定了一定的理论基础,具有良好的应用前景。
中文关键词: 核桃饼粕  分离蛋白  水解度  持水性  稳定性
Abstract:Walnuts are rich in fat,protein and healthy fiber.Its application attracted extensive attention.Walnut meal was the raw material.Protein isolate was extracted by acid precipitation and alkali extraction.Walnut protein with different degree of hydrolysis was prepared by alkaline protease.The water-holding capacity,emulsifying property and emulsion stability,foaming property and foam stability were measured.The result showed that the degree of hydrolysis reached a maximum of 19.4% at 120 min.The water-holding capacity and emulsifying property increased first and then decreased with the increase of enzymatic hydrolysis.The foaming property and emulsion stability increased with the increase of enzymatic hydrolysis degree.Foam stability decreased with the increase of enzymatic hydrolysis.It indicated that hydrolysis degree could improve or weaken some functional characteristics of enzymatic hydrolysate of protein of walnut.The results of the study laid a certain theoretical foundation for further utilization of walnut meal and had a good application prospect.
文章编号:202019005     中图分类号:    文献标志码:
基金项目:山西省高等学校科技创新项目(2019L0948);山西省青年科技研究基金(201901D211450);山西省重点实验室建设项目(201805D111012)
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