Abstract:In order to establish a simple，rapid and accurate detection method of bisphenol A，active ester method was used for coupling 4，4-bis（4-hydroxyphenyl）valeric acid（BVA）with bovine serum albumin and ovalbumin，respectively.BALB/c mice were immunized with the immune antigen to produce antibody.The BALB/c mice with high titer and sensitivity was chose to make hybridoma cell lines.After cell fusion with polyethylene glycol method，the hybridoma cell lines generating anti-BPA monoclonal antibodies were obtained.An indirect competitive enzyme-linked immunoassay for BPA was established by square titration and optimization of reaction conditions.The detection method had good linearity in 0.5 ng/mL-50 ng/mL，the minimum detection limit IC10was 0.5 ng/mL，the half inhibition rate IC50was 16.65 ng/mL，and the recovery rate of BPA in water was 89.72%-105.25%.This monoclonal antibody had high titer and specificity，this detection method had high sensitivity.